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KMID : 1134820190480070763
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 7 p.763 ~ p.768
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Optimization of Static and Dynamic Headspace Analyses of Trimethylamine Using Response Surface Methodology
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Hong Dong-Lee
Cho Sung-Rae
Yu Jeong-Wan
Kim So-Hyun
Kim Hyeon-Jae
Lee Yang-Bong
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Abstract
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Trimethylamine (TMA) is an index compound of fish smell in fishery products. The objective of this study is to optimize the static and dynamic headspace analyses with 200 μL direct injection and solid phase microextraction using response surface methodology. A fractional 4-level-3-factor experiment design which consists of 33 experimental trials and five replicates at nine center points was adopted to see the effect of independent values such as TMA concentration (2∼14 mg/100 g), sodium hydroxide concentration (5∼25%), temperature (4∼32°C), and time (3∼11 min) on the responses of isolated TMA amounts. Data were analyzed using SAS Statistical Software to get a second-order polynomial formula and the 3-dimensional graphs were drawn with Design-Expert Software. Regression coefficients (R2) of direct injection and solid phase microextraction were 0.9816 and 0.9802, respectively. TMA contents between static and dynamic analyses showed a similar tendency. These results will provide basic information in measuring TMA contents in fish products.
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KEYWORD
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trimethylamine, solid phase microextraction, headspace, response surface methodology
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